Collecting Good Hair Samples

Instructions for Collecting Good Hair Samples
Speckle Park Specific

As of January 1, 2011 all Speckle Park being registered with CSPA require a DNA Profile and must qualify against their sire before registration can be completed.  Other older animals may require a DNA Profile under certain circumstances as specified in the Association’s by-laws.  These DNA Profiles for Speckle Park are obtained from hair samples.  Here is what you need to know about collecting hair samples.

1.   Hair is pulled from the tail switch.

2.   Clean the area of the tail switch from which the hair is to be pulled.   Dirt, manure, urine and other foreign material will make the hair sample unfit for testing.  Brush any loose dirt or manure from the area.

3.   Secure 35 to 45 hairs from the tail switch with pliers or around your finger.  
DO NOT CLIP THE HAIR!  Pull the hair up towards the animal in the opposite direction the hair lies.
The hair follicles (the translucent bubls) on the end of the hair shaft must be present ffor successful testing.
If the hair follicles are small or hard to see, repeat the procedure and combine the hairs.
Additional hair follicles may be needed if you are requesting multiple tests in addition to testing for parentage verification.

4.   Put the group of hair follicles together and wrap a small piece of scotch tape around the bundle about 2 inches from the hair follicles.  DO NOT CURL THE HAIR IN THE BUNDLE.
The hair bundle may be further trimmed to 3 to 4 inches in length, but be sure to retain the end with the hair follicles.

5.   Tape the centre of the bundle of hair shafts to the middle of a piece of paper and then insert it into an envelope and seal it before collecting a sample from the next animal.
Any paper envelope letter size or smaller is acceptable.
Do not use plastic Ziploc bags because they have been known to retain moisture from the hair sample and ultimately render the sample unusable.

6.   Write clearly on the envelope to identify the animal.  Be sure to include the animal’s:
      *complete name
      *registration number, if available, and
       *ear tag.
This information ensures that your hair sample will be matched to the correct application and animal.

7.   If any DNA testing is to be done at a second laboratory, collect and retain a second hair sample.  Some breeders have adopted the practice of routinely take a second sample and retaining it with their Private Herd Records.

8.   Hair samples may now be submitted with the completed application to register your Specile Parkdirectly to CLRC with the applicqtion.  For this application here. This is a change in procedure adopted in mid-2016 in order to save time in the registration process.

If your animal is already registered and now needs a DNA profile or you wish a DNA profile without registering, please complete the Application for DNA Genetic Marker Tests form and submit the hair sample with the application to CLRC.  For a copy of application click here.  Be sure to follow the instructions.